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Bacteriostatic water is commonly used in order to dilute or dissolve substances such as reconstituting powdered substances for injection purposes. Bacteriostatic water is sterile water that contains 0.9% benzyl alcohol to prevent it from being contaminated by potential bacteria.
The antibacterial properties of the benzyl alcohol allow the bottle to be drawn from several times with a sterile needle. As a result of the addition of benzyl alcohol, the water is considered "static" because of the stability of the solution and unchanging bacteria content.
Bacteriostatic water can be used intravenously, intramuscularly, and subcutaneously depending on what the water is mixed with.
Despite being bacteria-free, bacteriostatic water is different from sterile, purified water since sterile, purified water has no additives. Bacteriostatic water is typically preferred by researchers over simply using an antibacterial agent because the addition of benzyl alcohol doesn’t just kill bacteria but prevents growth from happening long before it is used for research purposes. Other antibacterial agents can also react with medications potentially causing negative side effects or adverse reactions.
However, bacteriostatic water isn’t the primary choice in a few specific cases. For example, bacteriostatic water should not be used to treat newborns or pregnant women because the toxicity from the benzyl alcohol could potentially affect the fetus or baby. Bacteriostatic water should also not be used for hydration or any form of spinal anesthesia like an epidural. Bacteriostatic water shouldn’t be used on its own without an isotonic medication added because this can cause hemolysis.
The primary concern for peptide researchers today is product purity. Nord-sci guarantees our product purity by performing independent testing of our products and providing those certifications for our customers in our product descriptions.
THIS PRODUCT IS NOT INTENDED TO TREAT, CURE OR DIAGNOSE ANY CONDITION OR DISEASE AND IS NOT FOR HUMAN CONSUMPTION. ALL PRODUCTS OFFERED ARE INTENDED FOR LABORATORY RESEARCH USE ONLY.
Hospira Bacteriostatic Water is a sterile, non-pyrogenic diluent formulated for the reconstitution and dilution of research-grade lyophilized compounds in controlled laboratory environments. Preserved with 0.9% benzyl alcohol, this solution is designed as a multi-use laboratory vehicle, supporting repeat withdrawals from a single vial when appropriate aseptic technique is maintained.
Within peptide and small-molecule research workflows, bacteriostatic water is frequently used to prepare working solutions, adjust concentration ranges, and standardize administration volumes for in vitro and in vivo protocols. This product is supplied in a sealed, multiple-dose vial to streamline bench-level handling, inventory management, and reproducible experimental design.
Hospira Bacteriostatic Water – Research Specifications:
| Unit Size | 30 mL multi-dose vial (Bacteriostatic Water for Injection, USP) |
| Unit Quantity | 1 vial |
| Active Component | Sterile Water for Injection |
| Preservative | 0.9% benzyl alcohol added as a bacteriostatic agent |
| pH Range | Typically near neutral (USP-compliant range; see manufacturer packaging for lot-specific data) |
| Osmolality | Hypotonic solution prior to mixing with solutes |
| Appearance | Clear, colorless solution in sealed multi-dose vial |
| Source | Commercially manufactured sterile diluent (Hospira) |
| Storage Conditions | Store at controlled room temperature as indicated on the product label. Protect from excessive heat, direct light, and contamination. Follow manufacturer expiry and in-use dating guidance within laboratory SOPs. |
| Research Use Only | Provided solely as a laboratory reagent and diluent for research applications. Not for human or animal therapeutic use, injection, or diagnostic procedures. |
In a research setting, bacteriostatic water serves as a foundational utility reagent, enabling consistent reconstitution and dilution of lyophilized materials under standardized conditions. When paired with appropriate aseptic technique, a preserved multi-dose vial can support multiple draws for iterative experimental workflows, minimizing waste and improving process efficiency.
Typical laboratory use cases include:
Because Hospira Bacteriostatic Water contains benzyl alcohol as a preservative, it is often selected in research programs where multi-use vials are operationally preferred, and where protocol designers understand and control for the presence of a low-level preservative in the system being studied.
Reconstitution Guidelines (Research Use Only):
When using Hospira Bacteriostatic Water to reconstitute a lyophilized research compound, investigators should:
Note: This information is provided strictly as general laboratory guidance. All specific protocols, including volumes, schedules, and endpoints, must be defined by the research institution and aligned with internal quality, safety, and ethical frameworks.
In experimental planning, bacteriostatic water should be evaluated as an active component of the system, not a purely inert background, due to the presence of benzyl alcohol. Research teams typically consider:
Hospira Bacteriostatic Water is generally utilized where multi-use convenience and preserved sterility between withdrawals are prioritized, under the full control of experienced laboratory staff.
For robust, audit-ready research workflows, solvent and diluent traceability is as important as the traceability of active compounds. When using Hospira Bacteriostatic Water, best practices include:
THIS PRODUCT IS INTENDED FOR LABORATORY RESEARCH USE ONLY. NOT FOR HUMAN CONSUMPTION. NOT INTENDED TO DIAGNOSE, TREAT, CURE, OR PREVENT ANY DISEASE OR CONDITION. NOT FOR INJECTION OR ANY FORM OF BODILY INTRODUCTION IN HUMANS OR ANIMALS.
ALL ARTICLES AND PRODUCT INFORMATION PROVIDED ON THIS WEBSITE ARE FOR INFORMATONAL AND EDUCATIONAL PURPOSES ONLY.
The products offered on this website are furnished for in-vitro studies only. In-vitro studies (Latin: in glass) are performed outside of the body. These products are not medicines or drugs and have not been approved by the FDA to prevent, treat or cure any medical condition, ailment or disease. Bodily introduction of any kind into humans or animals is strictly forbidden by law.
